Since 2012, AMLD has worked to ensure that the duplication of the BR-101 highway, which crosses the tamarins' habitat, includes strategies to mitigate its impact on wildlife. AMLD participated in the licensing process to help environmental agencies identify strategic locations for wildlife passages.
After a broad mobilization campaign—which involved partners, supporters, a petition, and even a Public Civil Action forwarded by the Federal Public Ministry of Macaé—the highway concessionaire was obligated to build 10 treetop-to-canopy passages, 15 tunnels for terrestrial fauna, and a vegetated wildlife overpass. This overpass was the first of its kind on a federal highway in Brazil.
The overpass connects the Poço das Antas Biological Reserve to what is now the headquarters of the Golden Lion Tamarin Association, a protected area restored and managed by AMLD. The overpass was inaugurated in August 2020, but the planting was not completed until November 2021. AMLD is responsible for monitoring golden lion tamarins and maned sloths on the overpass, and it also registers any other species that may cross it.
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